PPP1CB Primary Antibody

Item Information
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Size
Price
Description

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed

Product Overview
Entrez GenelD
5500
Aliases
PP1B; PP-1B; PPP1CD; PP1beta
Clone#
6G10C1
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PPP1CB (AA: 174-327) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. J Biol Chem. 2011 Sep 23;286(38):32931-6.
2. Mol Biol Cell. 2010 Dec;21(24):4409-17.
Product Image
Western Blot
Figure 1: Western blot analysis using PPP1CB mAb against human PPP1CB (AA: 174-327) recombinant protein. (Expected MW is 43.2 kDa)
Figure 1: Western blot analysis using PPP1CB mAb against human PPP1CB (AA: 174-327) recombinant protein. (Expected MW is 43.2 kDa)
Western Blot
Figure 2: Western blot analysis using PPP1CB mAb against HEK293 (1) and PPP1CB (AA: 174-327)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using PPP1CB mAb against HEK293 (1) and PPP1CB (AA: 174-327)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using PPP1CB mouse mAb against Jurkat (1), A431 (2), Hela (3), HepG2 (4), HEK293 (5), MCF-7 (6) cell lysate.
Figure 3: Western blot analysis using PPP1CB mouse mAb against Jurkat (1), A431 (2), Hela (3), HepG2 (4), HEK293 (5), MCF-7 (6) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of Jurkat cells using PPP1CB mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of Jurkat cells using PPP1CB mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded liver tissues using PPP1CB mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded liver tissues using PPP1CB mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using PPP1CB mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using PPP1CB mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.