PPARGC1B Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene stimulates the activity of several transcription factors and nuclear receptors, including estrogen receptor alpha, nuclear respiratory factor 1, and glucocorticoid receptor. The encoded protein may be involved in fat oxidation, non-oxidative glucose metabolism, and the regulation of energy expenditure. This protein is downregulated in prediabetic and type 2 diabetes mellitus patients. Certain allelic variations in this gene increase the risk of the development of obesity. Three transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
133522
Aliases
PERC; ERRL1; PGC1B; PGC-1(beta)
Clone#
6C3F6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PPARGC1B (AA: 195-414) expressed in E. Coli.
Formulation
Purified antibody from tissue culture in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Am J Physiol Endocrinol Metab. 2011 Jul;301(1):E155-63.
2. J Biol Chem. 2009 Jul 24;284(30):19945-52.
2. J Biol Chem. 2009 Jul 24;284(30):19945-52.
Product Image
Western Blot
Figure 1: Western blot analysis using PPARGC1B mAb against human PPARGC1B (AA: 195-414) recombinant protein. (Expected MW is 50.3 kDa)
Western Blot
Figure 2: Western blot analysis using PPARGC1B mAb against HEK293 (1) and PPARGC1B (AA: 195-414)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using PPARGC1B mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.