PON1 Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | ESA; PON; MVCD5 |
| ProductOrder | P |
| Clone# | 4G8D3 |
| Entrez GenelD | 5444 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human PON1 (AA: 20-155) expressed in E. Coli. |
| MW | 39.7kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 20-155 | |
Western Blot
Figure 1: Western blot analysis using PON1 mAb against human PON1 recombinant protein. (Expected MW is 40.6 kDa)
Western Blot
Figure 2: Western blot analysis using PON1 mAb against HEK293 (1) and PON1 (AA: 20-155)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using PON1 mouse mAb against human plasma cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of Hela cells using PON1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PON1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PON1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);