PON1 Primary Antibody

Item Information

Catalog #

Size

Price

30572

100ul

$435.0

Description

The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3.

Product Overview

Entrez GenelD

5444

Aliases

ESA; PON; MVCD5

Clone#

4G8D3

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human PON1 (AA: 20-155) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Redox Rep. 2012;17(5):214-8.
2. Cancer Epidemiol. 2012 Apr;36(2):e101-3.

Product Image

Western Blot

Figure 1: Western blot analysis using PON1 mAb against human PON1 recombinant protein. (Expected MW is 40.6 kDa)

Western Blot

Figure 2: Western blot analysis using PON1 mAb against HEK293 (1) and PON1 (AA: 20-155)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 3: Western blot analysis using PON1 mouse mAb against human plasma cell lysate.

Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of Hela cells using PON1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunohistochemical analysis