POLR2A Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Product Overview
Entrez GenelD
5430
Aliases
RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
Clone#
1B1D8
Host / Isotype
Mouse / Mouse IgG2b
Immunogen
Purified recombinant fragment of human POLR2A (AA: 128-258) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Nat Genet. 2016 Oct;48(10):1253-9. 2.J Gen Virol. 2016 Jan;97(1):220-4.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using POLR2A mAb against human POLR2A (AA: 128-258) recombinant protein. (Expected MW is 41.1 kDa)
Western Blot
Figure 3:Western blot analysis using POLR2A mAb against HEK293 (1) and POLR2A (AA: 128-258)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometric
Figure 4:Flow cytometric analysis of Hela cells using POLR2A mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.