PODXL Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. (provided by RefSeq)Tissue specificity: Glomerular epithelium cell (podocyte)

Product Overview

Entrez GenelD

5420

Aliases

PC; PCLP; Gp200; PCLP-1; MGC138240

Clone#

5F5

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human PODXL expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Blood. 2009 Jan 22;113(4):816-26.
2. Am J Physiol Cell Physiol. 2009 Mar;296(3):C505-13.

Product Image

Western Blot

Figure 1: Western blot analysis using PODXL mAb against human PODXL (AA: 109-324) recombinant protein. (Expected MW is 47.3 kDa)

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney tissues (right) using PODXL mouse mAb with DAB staining.

Flow cytometric