PODXL Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesPC; PCLP; Gp200; PCLP-1; MGC138240
ProductOrderP
Clone#5F5
Entrez GenelD5420
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PODXL expressed in E. Coli.
MW49kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
109-324
Catalog#: 30206
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Images
Western Blot
Figure 1: Western blot analysis using PODXL mAb against human PODXL (AA: 109-324) recombinant protein. (Expected MW is 47.3 kDa)
Figure 1: Western blot analysis using PODXL mAb against human PODXL (AA: 109-324) recombinant protein. (Expected MW is 47.3 kDa)
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney tissues (right) using PODXL mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney tissues (right) using PODXL mouse mAb with DAB staining.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using PODXL mouse mAb (green) and negative control (purple).
Figure 3: Flow cytometric analysis of Hela cells using PODXL mouse mAb (green) and negative control (purple).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Product Overview
Description

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. (provided by RefSeq)Tissue specificity: Glomerular epithelium cell (podocyte)

References (references)
References (references)1. Blood. 2009 Jan 22;113(4):816-26.
2. Am J Physiol Cell Physiol. 2009 Mar;296(3):C505-13.