PMS2 Primary Antibody
The protein encoded by this gene is a key component of the mismatch repair system that functions to correct DNA mismatches and small insertions and deletions that can occur during DNA replication and homologous recombination. This protein forms heterodimers with the gene product of the mutL homolog 1 (MLH1) gene to form the MutL-alpha heterodimer. The MutL-alpha heterodimer possesses an endonucleolytic activity that is activated following recognition of mismatches and insertion/deletion loops by the MutS-alpha and MutS-beta heterodimers, and is necessary for removal of the mismatched DNA. There is a DQHA(X)2E(X)4E motif found at the C-terminus of the protein encoded by this gene that forms part of the active site of the nuclease. Mutations in this gene have been associated with hereditary nonpolyposis colorectal cancer (HNPCC; also known as Lynch syndrome) and Turcot syndrome. [provided by RefSeq, Apr 2016]
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31768ea_115.jpg?v=1666704504)
![Figure 2:Western blot analysis using PMS2 mAb against human PMS2 (AA: 748-851) recombinant protein. (Expected MW is 37.7 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31768rwb_116.jpg?v=1666704505)
![Figure 3:Western blot analysis using PMS2 mAb against HEK293 (1) and PMS2 (AA: 748-851)-hIgGFc transfected HEK293 (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31768twb_117.jpg?v=1666704505)
![Figure 4:Western blot analysis using PMS2 mouse mAb against Hela (1) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31768lwb_118.jpg?v=1666704505)
![Figure 5:Immunofluorescence analysis of Hela cells using PMS2 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31768icc-1_119.jpg?v=1666704505)
![Figure 6:Immunofluorescence analysis of Hela cells using PMS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31768icc-2_120.jpg?v=1666704505)
![Figure 7:Flow cytometric analysis of Hela cells using PMS2 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31768fcm_121.jpg?v=1666704505)