Mouse Monoclonal Antibody to PMEL
This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/97c4ff7330f5bf6473300e362c75fb464aa71d3f72a96dbde2baa606b4a3c731.jpg?v=1666705961)
![Figure 2:Western blot analysis using PMEL mAb against human PMEL (AA: 25-192) recombinant protein. (Expected MW is 44.7 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/0b2c7a9213cf8db26d2d7f8f02deb653c958a178be68763da7dcf86f1dc924cf.jpg?v=1666705961)
![Figure 3:Western blot analysis using PMEL mAb against HEK293-6e (1) and PMEL (AA: 25-192)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a406beeba56a06ec70326f4be7b6a4310305f73616c06b2d674df9d63e8ce023.jpg?v=1666705962)
![Figure 4:Flow cytometric analysis of HL-60 cells using PMEL mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/9c7f18bf930a140dfcdf1d06b5080474a143e22ba52ef748bb625d2e06841d18.jpg?v=1666705962)