PMAIP1 Primary Antibody
Item Information
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Size
Price
Description
The protein encoded by this gene promotes activation of caspases and apoptosis, Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.
Product Overview
Entrez GenelD
5366
Aliases
APR; NOXA
Clone#
1A2D3
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human PMAIP1 (AA: 1-54) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Oncotarget. 2014 Nov 30;5(22):11237-51. 2.Cell Death Dis. 2014 Jan 23;5:e1013.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using PMAIP1 mAb against human PMAIP1 (AA: 1-54) recombinant protein. (Expected MW is 32 kDa)
Western Blot
Figure 3:Western blot analysis using PMAIP1 mAb against HEK293 (1) and PMAIP1 (AA: 1-54)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometric
Figure 4:Flow cytometric analysis of Hela cells using PMAIP1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.