PLK1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Polo-like kinase 1 (Plk1) plays key roles in many aspects of mitosis. Suppression of Plk1 by p21(Waf1) is responsible for p53-dependent protection against adriamycin-induced caspase-independent mitotic death.
Product Overview
Entrez GenelD
5347
Aliases
PLK;STPK13
Clone#
3C11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human PLK1 (AA: 331-508) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cell Signal. 2011 Nov;23(11):1816-23. 2.Biochem Biophys Res Commun. 2011 Jun 24;410(1):57-61.
Product Image
Western Blot
Figure 1: Western blot analysis using PLK1 mAb against human PLK1 recombinant protein. (Expected MW is 45.7 kDa)
Western Blot
Figure 2: Western blot analysis using PLK1 mouse mAb against K562 (1) and Raji (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of NIH3T3 cells using PLK1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PLK1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PLK1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.