PLA2G7 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.

Product Overview

Entrez GenelD

7941

Aliases

PAFAD; PAFAH; LP-PLA2; LDL-PLA2

Clone#

3E1G5

Host / Isotype

Mouse / Mouse IgG1

Immunogen

Purified recombinant fragment of human PLA2G7 (AA: 22-441) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4?; -20? for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1.J Thromb Thrombolysis. 2018 Jul;46(1):125-130. 2.Diabetologia. 2018 May;61(5):1155-1166.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using PLA2G7 mAb against human PLA2G7 (AA: 22-441) recombinant protein. (Expected MW is 74.6 kDa)

Western Blot

Figure 3:Western blot analysis using PLA2G7 mAb against HEK293 (1) and PLA2G7 (AA: 22-441)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using PLA2G7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunohistochemical Analysis