PLA2G12A Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesGXII; ROSSY; PLA2G12
ProductOrderP
Clone#7C7C9
Entrez GenelD81579
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PLA2G12A (AA: 21-189) expressed in E. Coli.
MW21kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
21-189
Catalog#: 30628
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Images
Western Blot
Figure 1: Western blot analysis using PLA2G12A mAb against human PLA2G12A (AA: 21-189) recombinant protein. (Expected MW is 44.5 kDa)
Figure 1: Western blot analysis using PLA2G12A mAb against human PLA2G12A (AA: 21-189) recombinant protein. (Expected MW is 44.5 kDa)
Western Blot
Figure 2: Western blot analysis using PLA2G12A mAb against HEK293 (1) and PLA2G12A (AA: 21-189)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using PLA2G12A mAb against HEK293 (1) and PLA2G12A (AA: 21-189)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using PLA2G12A mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of Hela cells using PLA2G12A mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
DescriptionSecreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s
References (references)
References (references)1. J Biol Chem. 2003 Mar 21;278(12):10657-67.
2. Biochemistry. 2003 Oct 7;42(39):11494-503.