Pirh2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesARNIP; CHIMP; RNF199; RCHY1
ProductOrderP
Clone#1H10
Entrez GenelD25898
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human Pirh2 expressed in E. Coli.
MW30kDa; 60kDa (homodimer)
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Rat
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
(full)1-261
Catalog#: 30005
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Images
Western Blot
Figure 1: Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.
Figure 1: Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 mouse mAb
Figure 2: Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 mouse mAb
Flow cytometric
Figure 3: Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).
Figure 3: Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of Hela cells using Pirh2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 4: Immunofluorescence analysis of Hela cells using Pirh2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Overview
Description

Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.

References (references)
References (references)1. Biochem Biophys Res Commun. 2007 Dec 14;364(2):344-50.
2. J Natl Cancer Inst. 2004 Nov 17;96(22):1718-21.
3. Exp Cell Res. 2006 Oct 15;312(17):3370-8.
4. Cell. 2003 Mar 21;112(6):779-91.