Pirh2 Primary Antibody
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Description
Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.
Product Overview
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
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Western Blot
Figure 1: Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 mouse mAb
Flow cytometric
Figure 3: Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of Hela cells using Pirh2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
For Research Use Only. Not for use in diagnostic procedures.