PIK3R1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
Aliasesp85; GRB1; p85-ALPHA
ProductOrderP
Clone#6G10
Entrez GenelD5295
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PIK3R1 (AA: 159-388) expressed in E. Coli.
MW83.6kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/100
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
159-388
Catalog#: 30402
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Contact us to order
1-866-339-0871
510-740-3625
info@promab.com
Images
Western Blot
Figure 1: Western blot analysis using PIK3R1 mAb against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)
Figure 1: Western blot analysis using PIK3R1 mAb against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of HeLa cells using PIK3R1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 2: Immunofluorescence analysis of HeLa cells using PIK3R1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using PIK3R1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of HepG2 cells using PIK3R1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of NIH3T3 cells using PIK3R1 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of NIH3T3 cells using PIK3R1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PIK3R1 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PIK3R1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PIK3R1 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PIK3R1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.

References (references)
References (references)1.Cancer Epidemiol Biomarkers Prev. 2011 May;20(5):923-33. 2.Cancer Res. 2010 Jul 1;70(13):5305-15.
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