Phospho-4E-BP1 (Ser65) Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Product Overview
Entrez GenelD
1978
Aliases
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
Clone#
1E2E7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Synthesized peptide of human Phospho-4E-BP1 (Ser65).
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/100 - 1/500
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Sci Signal. 2015 Nov 17;8(403):ra116.
2.Oncotarget. 2015 Sep 15;6(27):24092-104.
2.Oncotarget. 2015 Sep 15;6(27):24092-104.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Immunofluorescence analysis
Figure 2:Immunofluorescence analysis of Hela cells using Phospho-4E-BP1 (Ser65) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 3:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.
Flow cytometric
Figure 5:Flow cytometric analysis of Jurkat cells using Phospho-4E-BP1 (Ser65) mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.