Phospho-4E-BP1 (Ser65) Primary Antibody
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Immunofluorescence analysis of Hela cells using Phospho-4E-BP1 (Ser65) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 3:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.
Figure 4:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.
Figure 5:Flow cytometric analysis of Jurkat cells using Phospho-4E-BP1 (Ser65) mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.