PHB Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesPHB1
ProductOrderP
Clone#5H7
Entrez GenelD5245
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PHB expressed in E. Coli.
MW30kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse, Rat, Monkey
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
68-259
Catalog#: 30264
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Images
Western Blot
Figure 1: Western blot analysis using PHB mAb against human PHB (AA: 68-259) recombinant protein. (Expected MW is 46.7 kDa)
Figure 1: Western blot analysis using PHB mAb against human PHB (AA: 68-259) recombinant protein. (Expected MW is 46.7 kDa)
Western Blot
Figure 2: Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.
Figure 2: Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PHB mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PHB mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PHB mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PHB mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PHB mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PHB mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PHB mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PHB mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 7: Immunofluorescence analysis of NIH/3T3 cells using PHB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 7: Immunofluorescence analysis of NIH/3T3 cells using PHB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 8: Flow cytometric analysis of MCF-7 cells using PHB mouse mAb (blue) and negative control (red).
Figure 8: Flow cytometric analysis of MCF-7 cells using PHB mouse mAb (blue) and negative control (red).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Product Overview
Description

Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.

References (references)
References (references)1. Biochem Biophys Res Commun. 2009 Dec 18;390(3):1023-8.
2. J Cell Biochem. 2009 Nov 1;108(4):926-34.