PGA5 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesPepsinogen 5; Pepsin A
ProductOrderP
Clone#2C1
Entrez GenelD5222
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PGA5 expressed in E. Coli.
MW42kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
203-307
Catalog#: 20396
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Images
Western Blot
Figure 1: Western blot analysis using PGA5 mouse mAb against HepG2 (1) and SMMC-7721 (2) cell lysate.
Figure 1: Western blot analysis using PGA5 mouse mAb against HepG2 (1) and SMMC-7721 (2) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PGA5 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PGA5 mouse mAb with DAB staining.
Product Overview
Description

PGA5: Pepsinogen 5, group I (pepsinogen A). Pepsinogens are the inactive precursors of pepsin, the major acid protease found in the stomach. Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. PGA5 is a member of the subfamily A1 within the pepsin family and is the predominant endopeptidase in the gastric juice of vertebrates. PGA5 is inhibited by ovUS-1, a uterine serpin.

References (references)
References (references)1. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16899-903.
2. Nat Genet. 2004 Jan;36(1):40-5.