PER3 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known.
Product Overview
Entrez GenelD
8863
Aliases
GIG13
Clone#
4B9D7
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PER3 (AA: 723-954) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Liver Int. 2012 Oct;32(9):1451-9.
2. Ann Surg Oncol. 2012 Sep;19(9):3081-8.
2. Ann Surg Oncol. 2012 Sep;19(9):3081-8.
Product Image
Western Blot
Figure 1: Western blot analysis using PER3 mAb against human PER3 (AA: 723-954) recombinant protein. (Expected MW is 50.7 kDa)
Western Blot
Figure 2: Western blot analysis using PER3 mAb against HEK293 (1) and PER3 (AA: 723-954)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3:Immunofluorescence analysis of Hela cells using PER3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using PER3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PER3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PER3 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.