PDPK1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesPDK1; PDPK2; PDPK2P; PRO0461
ProductOrderP
Clone#7E4G11
Entrez GenelD5170
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PDPK1 (AA: 457-556) expressed in E. Coli.
MW63.2kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse, Monkey, Rat
Application
ELISA1/10000
ICC (Immunocytochemistry)1/50 - 1/200
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
457-556
Catalog#: 30805
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using PDPK1 mAb against human PDPK1 (AA: 457-556) recombinant protein. (Expected MW is 37.9 kDa)
Figure 2:Western blot analysis using PDPK1 mAb against human PDPK1 (AA: 457-556) recombinant protein. (Expected MW is 37.9 kDa)
Western Blot
Figure 3:Western blot analysis using PDPK1 mAb against HEK293 (1) and PDPK1 (AA: 457-556)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using PDPK1 mAb against HEK293 (1) and PDPK1 (AA: 457-556)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using PDPK1 mouse mAb against MCF-7 (1), Hela (2), K562 (3), U937 (4), A549 (5), NIH/3T3 (6), Jurkat (7), PC-12 (8), and Cos7 (9) cell lysate.
Figure 4:Western blot analysis using PDPK1 mouse mAb against MCF-7 (1), Hela (2), K562 (3), U937 (4), A549 (5), NIH/3T3 (6), Jurkat (7), PC-12 (8), and Cos7 (9) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of A549 cells using PDPK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of A549 cells using PDPK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of A549 cells using PDPK1 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of A549 cells using PDPK1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using PDPK1 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using PDPK1 mouse mAb with DAB staining.
Product Overview
DescriptionPhosphoinositide-dependent kinase 1 (PDPK1, PDK1) is a serine/threonine protein kinase integral to the function of the PI 3-K/Akt signaling pathway. PDK1 and mTORC2 both phosphorylate and activate PKB/Akt, ensuring a cellular response to stimuli such as growth factors and insulin signaling. Akt is the main effector of PDK1.
References (references)
References (references)1.Asian Pac J Cancer Prev. 2012;13(8):4147-51.
2.Mol Cancer Res. 2010 Mar;8(3):421-32.