PDPK1 Primary Antibody
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Description
Phosphoinositide-dependent kinase 1 (PDPK1, PDK1) is a serine/threonine protein kinase integral to the function of the PI 3-K/Akt signaling pathway. PDK1 and mTORC2 both phosphorylate and activate PKB/Akt, ensuring a cellular response to stimuli such as growth factors and insulin signaling. Akt is the main effector of PDK1.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using PDPK1 mAb against human PDPK1 (AA: 457-556) recombinant protein. (Expected MW is 37.9 kDa)
Western Blot
Figure 3:Western blot analysis using PDPK1 mAb against HEK293 (1) and PDPK1 (AA: 457-556)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using PDPK1 mouse mAb against MCF-7 (1), Hela (2), K562 (3), U937 (4), A549 (5), NIH/3T3 (6), Jurkat (7), PC-12 (8), and Cos7 (9) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of A549 cells using PDPK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of A549 cells using PDPK1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using PDPK1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.