Mouse Monoclonal Antibody to PDLIM7

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Description

The protein encoded by this gene is representative of a family of proteins composed of conserved PDZ and LIM domains. LIM domains are proposed to function in protein-protein recognition in a variety of contexts including gene transcription and development and in cytoskeletal interaction. The LIM domains of this protein bind to protein kinases, whereas the PDZ domain binds to actin filaments. The gene product is involved in the assembly of an actin filament-associated complex essential for transmission of ret/ptc2 mitogenic signaling. The biological function is likely to be that of an adapter, with the PDZ domain localizing the LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Alternative splicing of this gene results in multiple transcript variants.

Product Overview
Entrez GenelD
9260
Aliases
LMP1; LMP3
Clone#
3B3B3
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human PDLIM7 (AA: 86-280) expressed in HEK293-6e cells supernatant.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Med Sci Monit. 2018 Oct 25;24:7603-7613. 2.Oncol Rep. 2015 May;33(5):2253-60.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using PDLIM7 mAb against human PDLIM7 (AA: 86-280) recombinant protein. (Expected MW is 51.2 kDa)
Figure 2:Western blot analysis using PDLIM7 mAb against human PDLIM7 (AA: 86-280) recombinant protein. (Expected MW is 51.2 kDa)
Immunofluorescence analysis
Figure 3:Immunofluorescence analysis of Hela cells using PDLIM7 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Figure 3:Immunofluorescence analysis of Hela cells using PDLIM7 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using PDLIM7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using PDLIM7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Jurkat cells using PDLIM7 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Jurkat cells using PDLIM7 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PDLIM7 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PDLIM7 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PDLIM7 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PDLIM7 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.