PDK2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Product Overview
Entrez GenelD
5164
Aliases
PDHK2; PDKII
Clone#
3F2D7
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PDK2 (AA: 178-404) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. J Biol Chem. 2006 May 5;281(18):12568-79
2. Biochemistry. 2004 Oct 26;43(42):13442-51.
2. Biochemistry. 2004 Oct 26;43(42):13442-51.
Product Image
Western Blot
Figure 1: Western blot analysis using PDK2 mAb against human PDK2 (AA: 178-404) recombinant protein. (Expected MW is 51.2 kDa)
Western Blot
Figure 2: Western blot analysis using PDK2 mAb against HEK293 (1) and PDK2 (AA: 178-404)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using PDK2 mouse mAb against Jurkat (1), C6 (2), Cos7 (3), K562 (4), A431 (5) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of A431 cells using PDK2 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.