PDK2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Product Overview

Entrez GenelD

5164

Aliases

PDHK2; PDKII

Clone#

3F2C4

Host / Isotype

Mouse / IgG2b

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human PDK2 (AA: 178-404) expressed in E. Coli.

Formulation

Purified antibody from tissue culture in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Biochemistry. 2008 Feb 26;47(8):2298-311.
2. J Biol Chem. 2006 May 5;281(18):12568-79.

Product Image

Western Blot

Figure 1: Western blot analysis using PDK2 mAb against human PDK2 (AA: 178-404) recombinant protein. (Expected MW is 51.2 kDa)

Western Blot

Figure 2: Western blot analysis using PDK2 mAb against HEK293 (1) and PDK2 (AA: 178-404)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of Hela cells using PDK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric