PDGFRA Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCD140A; PDGFR2; PDGFR-2; RHEPDGFRA
ProductOrderP
Clone#8E12F2
Entrez GenelD5156
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PDGFRA (AA: 361-528) expressed in E. Coli.
MW122.7kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
361-528
Catalog#: 30728
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Images
Western Blot
Figure 1: Western blot analysis using PDGFRA mAb against human PDGFRA (AA: 361-528) recombinant protein. (Expected MW is 44.8 kDa)
Figure 1: Western blot analysis using PDGFRA mAb against human PDGFRA (AA: 361-528) recombinant protein. (Expected MW is 44.8 kDa)
Western Blot
Figure 2: Western blot analysis using PDGFRA mAb against HEK293 (1) and PDGFRA (AA: 361-528)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using PDGFRA mAb against HEK293 (1) and PDGFRA (AA: 361-528)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3:Immunofluorescence analysis of Hela cells using PDGFRA 8E12F2 HELA 100 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 3:Immunofluorescence analysis of Hela cells using PDGFRA 8E12F2 HELA 100 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using PDGFRA mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of Hela cells using PDGFRA mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.

References (references)
References (references)1. Stem Cells Dev. 2013 Jul 1;22(13):1932-43.
2. Cancer Biol Ther. 2012 Dec;13(14):1384-9.