PD1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
Product Overview
Entrez GenelD
5133
Aliases
PDCD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Clone#
3B3D9
Host / Isotype
Mouse / Mouse IgG2b
Immunogen
Purified recombinant fragment of human PD1 (AA: 192-288) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Am J Surg Pathol. 2018 Jul;42(7):843-854. 2.PLoS One. 2018 Feb 28;13(2):e0192704.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using PD1 mAb against human PD1 (AA: 192-288) recombinant protein. (Expected MW is 36.5 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using PD1 mAb against HEK293 (1) and PD1 (AA: 192-288)-hIgGFc transfected HEK293 (2) cell lysate.
WESTERN BLOT
Figure 4: Western blot analysis using PD1 mouse mAb against Jurkat (1), U937 (2), and Hek293 (3) cell lysate.
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of Hela cells using PD1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.