PD1 Primary Antibody

Item Information
Catalog #
Size
Price
Description
This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
Product Overview
Entrez GenelD
5133
Aliases
PDCD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Clone#
1D6F6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PD1 (AA: extra 21-170) expressed in HEK293 cells.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2016 Jul 26;11(7):e0159383.
2.Tumour Biol. 2016 Jun;37(6):7507-14.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using PD1 mAb against human PD1 (AA: extra 21-170) recombinant protein. (Expected MW is 46.6 kDa)
Figure 2:Western blot analysis using PD1 mAb against human PD1 (AA: extra 21-170) recombinant protein. (Expected MW is 46.6 kDa)
Western Blot
Figure 3:Western blot analysis using PD1 mouse mAb against MOLT4 (1), K562 (2), Ramos (3), HL-60 (4), THP-1 (5), and U937 (6) cell lysate.
Figure 3:Western blot analysis using PD1 mouse mAb against MOLT4 (1), K562 (2), Ramos (3), HL-60 (4), THP-1 (5), and U937 (6) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using PD1 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using PD1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.