PD1 Primary Antibody

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Description
This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using PD1 mAb against human PD1 (AA: extra 21-170) recombinant protein. (Expected MW is 46.6 kDa)
Figure 2:Western blot analysis using PD1 mAb against human PD1 (AA: extra 21-170) recombinant protein. (Expected MW is 46.6 kDa)
Immunofluorescence analysis
Figure 3:Immunofluorescence analysis of Hela cells using PD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 3:Immunofluorescence analysis of Hela cells using PD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using PD1 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of HL-60 cells using PD1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.