PD1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.

Product Overview

Entrez GenelD

5133

Aliases

PDCD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1

Clone#

3E12A10

Host / Isotype

Mouse / IgG2a

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human PD1 (AA: extra 21-170) expressed in HEK293 cells.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.PLoS One. 2016 Jul 26;11(7):e0159383.
2.Tumour Biol. 2016 Jun;37(6):7507-14.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using PD1 mAb against human PD1 (AA: extra 21-170) recombinant protein. (Expected MW is 46.6 kDa)

Immunofluorescence analysis

Figure 3:Immunofluorescence analysis of Hela cells using PD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric