PCDH9 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the protocadherin family, and cadherin superfamily, of transmembrane proteins containing cadherin domains. These proteins mediate cell adhesion in neural tissues in the presence of calcium. The encoded protein may be involved in signaling at neuronal synaptic junctions. Sharing a characteristic with other protocadherin genes, this gene has a notably large exon that encodes multiple cadherin domains and a transmembrane region. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Product Overview

Entrez GenelD

5101

Aliases

Clone#

7G3A2

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human PCDH9 (AA: 24-148) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/50 - 1/250

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Clin Exp Metastasis. 2015 Jun;32(5):417-28.
2.J Clin Neurosci. 2012 Apr;19(4):541-5.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using PCDH9 mAb against human PCDH9 (AA: 24-148) recombinant protein. (Expected MW is 40 kDa)

Western Blot

Figure 3:Western blot analysis using PCDH9 mAb against HEK293 (1) and PCDH9 (AA: 24-148)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using PCDH9 mouse mAb against C6 (1) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using PCDH9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric