PBK Primary Antibody
Item Information
Catalog #
Size
Price
Description
This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.
Product Overview
Entrez GenelD
55872
Aliases
SPK; CT84; TOPK; Nori-3
Clone#
2C8
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PBK expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
Cancer Res. 2007 Jun 1;67(11):5186-94
Cancer Sci. 2010 Feb;101(2):403-11
Cancer Sci. 2010 Feb;101(2):403-11
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2: Western blot analysis using PBK mAb against human PBK (AA: 50-230) recombinant protein. (Expected MW is 45.8 kDa)
Western Blot
Figure 3: Western blot analysis using PBK mouse mAb against A431 (1) cell lysate.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PBK mouse mAb with DAB staining.
Immunohistochemical analysis
Figure5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PBK mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 6: Immunofluorescence analysis of Hela cells using PBK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 7: Flow cytometric analysis of Hela cells using PBK mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.