PAX8 Primary Antibody
Item Information
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Size
Price
Description
This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]
Product Overview
Entrez GenelD
7849
Aliases
pax8
Clone#
3E6B3
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human PAX8 (AA: 300-450) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Oncogene. 2019 Oct;38(40):6696-6710.2.Nat Commun. 2019 Aug 20;10(1):3739.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using PAX8 mAb against human PAX8 (AA: 300-450) recombinant protein. (Expected MW is 18.6 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using PAX8 mAb against HEK293 (1) and PAX8 (AA: 300-450)-hIgGFc transfected HEK293 (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of SK-OV-3 cells using PAX8 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.