PAX5 Primary Antibody

Item Information
Catalog #
Size
Price
Description

This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined.

Product Overview
Entrez GenelD
5079
Aliases
BSAP
Clone#
7D3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PAX5 (AA: 235-382) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1.Cancer Res. 2011 Dec 15;71(24):7345-50. 2.Am J Clin Pathol. 2010 Jan;133(1):41-8.
Product Image
Western Blot
Figure 1: Western blot analysis using PAX5 mAb against human PAX5 recombinant protein. (Expected MW is 41.2 kDa)
Figure 1: Western blot analysis using PAX5 mAb against human PAX5 recombinant protein. (Expected MW is 41.2 kDa)
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded brain tumors tissues using PAX5 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded brain tumors tissues using PAX5 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using PAX5 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using PAX5 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.