PAX2 Primary Antibody
Item Information
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Price
Description
PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor suppressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Product Overview
Entrez GenelD
5076
Aliases
FSGS7; PAPRS
Clone#
2C8C6
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human PAX2 (AA: 194-304) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Endocr Relat Cancer. 2018 Dec 1;25(12):981-991.2.J Cell Physiol. 2019 Mar;234(3):2929-2942.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using PAX2 mAb against human PAX2 (AA: 194-304) recombinant protein. (Expected MW is 27.5 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using PAX2 mAb against HEK293 (1) and PAX2 (AA:194-304)-hIgGFc transfected HEK293 (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of Hela cells using PAX2 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.