PAK2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
PAK2, also known as P21 (CDKN1A)-activated kinase 2. The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. PAK2 is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. PAK2 has been shown to interact with SH3KBP1, CDC42 and Abl gene.
Product Overview
Entrez GenelD
5062
Aliases
PAK65; PAKgamma
Clone#
3B5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Monkey
Immunogen
Purified recombinant fragment of PAK2 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. J Immunol. 2004 Jun 15;172(12):7324-34.
2. J Mol Biol. 2007 Jul 20;370(4):620-32.
2. J Mol Biol. 2007 Jul 20;370(4):620-32.
Product Image
Western Blot
Figure 1: Western blot analysis using PAK2 mouse mAb against Hela (1), Jurkat (2), A549 (3), HEK293 (4) and K562 (5) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and gastric cancer (right) using PAK2 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Confocal Immunofluorescence analysis of Hela cells using PAK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
For Research Use Only. Not for use in diagnostic procedures.