Mouse Monoclonal Antibody to P4HB
This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008]
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/da49facfa24b3fe4287b4ea07544075a7e833bcf4e523c00bdc1ee753319000a.jpg?v=1666706064)
![Figure 3:Western blot analysis using P4HB mouse mAb against Hela (1), PANC-1 (2), MCF-7 (3),THP-1 (4),SW620 (5)and HepG2(6) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f81cc610ec232651e7efc38dd88b69103b2d907dcc9745af06a378383f0116e6.jpg?v=1666706064)
![Figure 4:Immunofluorescence analysis of Hela cells using P4HB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/04f60f2938dc6fadfb98c8b05557211bc5e748c6a798023d5259c5455fb074ff.jpg?v=1666706064)
![Figure 5:Flow cytometric analysis of Hela cells using P4HB mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3015014ff669b26789c06158759cf081dbe3e90e4f979ea8ce5218d5b0d46d72.jpg?v=1666706064)
![Figure 6:Flow cytometric analysis of HepG2 cells using P4HB mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/1252a2a3ec913f0fe3e37620b8ccd239a303149c07c4d67e6c1bf22cabadf3cf.jpg?v=1666706064)
![Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using P4HB mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/b129f0855f0ca8c54562ab8064d7525fe060a3725fefac68c66889b8b0d9ce5e.jpg?v=1666706064)
![Figure 8:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using P4HB mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c4e9eac73bd9b58be1d894502cf1cf1f2c75a64fc62d94999c87ae9b08274065.jpg?v=1666706064)
![Figure 9:Western blot analysis using P4HB mAb against human P4HB (AA: 309-508) recombinant protein. (Expected MW is 53.5 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/4a8e7d420ba661d3c7f59ed55dc197f4859c6649871659eeda1408e39cf1bea2.jpg?v=1666706064)