Mouse Monoclonal Antibody to P4HB
This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c4bc5e8009db056a8091e304a10fddf4b1fa3678209870bed550264d4ed6eede.jpg?v=1666705898)
![Figure 2:Western blot analysis using P4HB mAb against human P4HB (AA: 309-508) recombinant protein. (Expected MW is 53.5 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/39d57024c3627eef6519af4e70df8ee4f9135a58b9a6887fa749f32630dff14a.jpg?v=1666705898)
![Figure 3:Western blot analysis using P4HB mouse mAb against Hela (1), PANC-1 (2), MCF-7 (3), THP-1 (4), SW620 (5), and HepG2 (6) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/59f8bb76b278450bab7c44bd54c341c0830ae77b24ebebc5c82535b12ee0a0ba.jpg?v=1666705898)
![Figure 4:Flow cytometric analysis of A375 cells using P4HB mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/b605e3fdeb85b3f27838ec620b5b3cca4a91b56af63df4f70fb08abe05e9e270.jpg?v=1666705898)
![Figure 5:Flow cytometric analysis of Hela cells using P4HB mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/85be242994351264f476e2f45eff84a9341c3887a5128186a0212c08e3733248.jpg?v=1666705898)
![Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using P4HB mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3e0c1a11ca41f2729423b5b3ca12be07944df27e065753ffda0c4ae27c962492.jpg?v=1666705898)
![Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using P4HB mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/7620a8597bdacc82a02eea6288b70b5a9ec4dda4baea30527aa8c3833c8a5ab3.jpg?v=1666705898)
![Figure 8:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using P4HB mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3ec8e48e6cdc4964a09b33cddad28210f4ddf632e8019c254d966147bca57a3d.jpg?v=1666705898)
![Figure 9:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using P4HB mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ec7e608439479c3496d4911c5ed598bca1dd12269f588ae0e22b1c3d21d18d06.jpg?v=1666705898)