P4HB

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesDSI,GIT, PDI,PHDB, PDIA1,PO4DB, PO4HB,PROHB,CLCRP1, ERBA2L,P4Hbeta
Clone#4E10C6F6
Entrez GenelD5034
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG2a
ImmunogenPurified recombinant fragment of human P4HB (AA:309-508) expressed in mammalian.
MW57.1kDa
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
309-508
Catalog#: 32272
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 3:Western blot analysis using P4HB mouse mAb against Hela (1), PANC-1 (2), MCF-7 (3),THP-1 (4),SW620 (5)and HepG2 (6)cell lysate.
Figure 3:Western blot analysis using P4HB mouse mAb against Hela (1), PANC-1 (2), MCF-7 (3),THP-1 (4),SW620 (5)and HepG2 (6)cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using P4HB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using P4HB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of HepG2 cells using P4HB mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of HepG2 cells using P4HB mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using P4HB mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using P4HB mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using P4HB mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using P4HB mouse mAb with DAB staining.
Western Blot
Figure 9:Western blot analysis using P4HB mAb against human P4HB (AA: 309-508) recombinant protein. (Expected MW is 53.5 kDa)
Figure 9:Western blot analysis using P4HB mAb against human P4HB (AA: 309-508) recombinant protein. (Expected MW is 53.5 kDa)
Product Overview
Description

This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

References (references)
References (references)1.mBio.2020 Jan 28;11(1):e03141-19.
2.Elife.2020 May 28;9:e54612.