P4HB
This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
2.Elife.2020 May 28;9:e54612.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32272ea.jpg?v=1669029455)
![Figure 3:Western blot analysis using P4HB mouse mAb against Hela (1), PANC-1 (2), MCF-7 (3),THP-1 (4),SW620 (5)and HepG2 (6)cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32272lwb.jpg?v=1669029455)
![Figure 4:Immunofluorescence analysis of Hela cells using P4HB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32272icc.jpg?v=1669029455)
![Figure 6:Flow cytometric analysis of HepG2 cells using P4HB mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32272-1fcm.jpg?v=1669029455)
![Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using P4HB mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32272ihc.jpg?v=1669029455)
![Figure 8:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using P4HB mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32272-1ihc.jpg?v=1669029455)
![Figure 9:Western blot analysis using P4HB mAb against human P4HB (AA: 309-508) recombinant protein. (Expected MW is 53.5 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32272rwb.jpg?v=1669029455)