P2RY8 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene belongs to the family of G-protein coupled receptors, that are preferentially activated by adenosine and uridine nucleotides. This gene is moderately expressed in undifferentiated HL60 cells, and is located on both chromosomes X and Y.
Product Overview
Entrez GenelD
286530
Aliases
P2Y8
Clone#
1G5A2
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human P2RY8 (AA: extra mix) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/100 - 1/500
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Exp Med. 2015 Dec 14;212(13):2213-22.
2.Blood. 2010 Jul 1;115(26):5393-7.
2.Blood. 2010 Jul 1;115(26):5393-7.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using P2RY8 mAb against human P2RY8 (AA: extra mix) recombinant protein. (Expected MW is 6.6 kDa)
Western Blot
Figure 3:Western blot analysis using P2RY8 mAb against HEK293 (1) and P2RY8 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using P2RY8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using P2RY8 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using P2RY8 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.