P2RX7 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria.
Product Overview
Entrez GenelD
5027
Aliases
P2X7
Clone#
7G1D6
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human P2RX7 (AA: 226-452) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Sci. 2015 Sep;106(9):1224-31.
2.J Hypertens. 2013 Dec;31(12):2362-9.
2.J Hypertens. 2013 Dec;31(12):2362-9.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using P2RX7 mAb against human P2RX7 (AA: 226-452) recombinant protein. (Expected MW is 48.9 kDa)
Western Blot
Figure 3:Western blot analysis using P2RX7 mAb against HEK293 (1) and P2RX7 (AA: 226-452)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using P2RX7 mouse mAb against A431 (1), U251 (2), Hela (3), U937 (4), and HepG2 (5) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using P2RX7 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using P2RX7 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.