OTUB2
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | OTB2; OTU2; C14orf137 |
| Clone# | 2B7C4 |
| Entrez GenelD | 78990 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Immunogen | Purified recombinant fragment of human OTUB2 (AA: full(1-234)) expressed in E. Coli. |
| MW | 27KDa |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| full(1-234) | |
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using OTUB2 mAb against human OTUB2 (AA: full(1-234)) recombinant protein. (Expected MW is 30 kDa)
Western Blot
Figure 3:Western blot analysis using OTUB2 mAb against HEK293-6e (1) and OTUB2 (AA: full(1-234))-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using OTUB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hela cells using OTUB2 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of HepG2 cells using OTUB2 mouse mAb (green) and negative control (red).