OTUB2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain.
Immunogen:

Product Overview

Entrez GenelD

78990

Aliases

OTB2; OTU2; C14orf137

Clone#

2B7C4

Host / Isotype

Mouse / Mouse IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human OTUB2 (AA: full(1-234)) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Cell Transplant. Jan-Dec 2020;29:963689720931433.
2.Mol Cell. 2014 Feb 20;53(4):617-30.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using OTUB2 mAb against human OTUB2 (AA: full(1-234)) recombinant protein. (Expected MW is 30 kDa)

Western Blot

Figure 3:Western blot analysis using OTUB2 mAb against HEK293-6e (1) and OTUB2 (AA: full(1-234))-hIgGFc transfected HEK293-6e (2) cell lysate.

Immunohistochemical analysis

Figure 4:Immunofluorescence analysis of Hela cells using OTUB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis