OTUB2

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesOTB2; OTU2; C14orf137
Clone#2B7C4
Entrez GenelD78990
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG1
ImmunogenPurified recombinant fragment of human OTUB2 (AA: full(1-234)) expressed in E. Coli.
MW27KDa
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
full(1-234)
Catalog#: 32280
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using OTUB2 mAb against human OTUB2 (AA: full(1-234)) recombinant protein. (Expected MW is 30 kDa)
Figure 2:Western blot analysis using OTUB2 mAb against human OTUB2 (AA: full(1-234)) recombinant protein. (Expected MW is 30 kDa)
Western Blot
Figure 3:Western blot analysis using OTUB2 mAb against HEK293-6e (1) and OTUB2 (AA: full(1-234))-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using OTUB2 mAb against HEK293-6e (1) and OTUB2 (AA: full(1-234))-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using OTUB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using OTUB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hela cells using OTUB2 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using OTUB2 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of HepG2 cells using OTUB2 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of HepG2 cells using OTUB2 mouse mAb (green) and negative control (red).
Product Overview
DescriptionThis gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain.
Immunogen:
References (references)
References (references)1.Cell Transplant. Jan-Dec 2020;29:963689720931433.
2.Mol Cell. 2014 Feb 20;53(4):617-30.