OTUB2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain.
Product Overview
Entrez GenelD
78990
Aliases
OTB2; OTU2; C14orf137
Clone#
2B7E7
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human OTUB2 (AA:full(1-234)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cell Transplant. Jan-Dec 2020;29:963689720931433.
2.Mol Cell. 2014 Feb 20;53(4):617-30.
2.Mol Cell. 2014 Feb 20;53(4):617-30.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using OTUB2 mAb against human OTUB2 (AA: full(1-234)) recombinant protein. (Expected MW is 30 kDa)
Western Blot
Figure 4:Western blot analysis using OTUB2 mouse mAb against HepG2 (1), A549 (2),HT-29 (3), MCF-7 (4), Jurkat (5), Hela (6), and Hek293 (7) cell lysate.
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hela cells using OTUB2 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of HepG2 cells using OTUB2 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.