ONECUT3 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a transcriptional activator. It can bind the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR(By similarity).
Product Overview
Entrez GenelD
390874
Aliases
OC3
Clone#
7D4G10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ONECUT3 (AA: 350-455) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Biochem Biophys Res Commun. 2002 Apr 12;292(4):848-54.
Product Image
Western Blot
Figure 1: Western blot analysis using ONECUT3 mAb against human ONECUT3 recombinant protein. (Expected MW is 38.2 kDa)
Western Blot
Figure 2: Western blot analysis using ONECUT3 mAb against HEK293 (1) and ONECUT3 (AA: 350-455)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of A431 cells using ONECUT3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ONECUT3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ONECUT3 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.