Oct4 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesOCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4; MGC22487; POU5F1
ProductOrderO
Clone#7E7
Entrez GenelD5460
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenSynthesized peptide derived from internal of human Oct4.
MW45kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
CPVSVTTLGSPMHSN
Catalog#: 30119
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Images
Western Blot
Figure 1: Western blot analysis using Oct4 mouse mAb against NTERA-2 (1) cell lysate.
Figure 1: Western blot analysis using Oct4 mouse mAb against NTERA-2 (1) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of NTERA-2 cells using Oct4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 2: Immunofluorescence analysis of NTERA-2 cells using Oct4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 3: Flow cytometric analysis of Jurkat cells using Oct4 mouse mAb (green) and negative control (purple).
Figure 3: Flow cytometric analysis of Jurkat cells using Oct4 mouse mAb (green) and negative control (purple).
Product Overview
Description

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. (provided by RefSeq).Tissue specificity: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

References (references)
References (references)1. Stem Cells. 2010 May;28(5):885-93.
2. Mol Med. 2010 Jul-Aug;16(7-8):247-53.
3. Med Sci (Paris). 2010 Apr;26(4):411-6.