Nucleophosmin Primary Antibody
Item Information
Catalog #
Size
Price
Description
Nucleophosmin (NPM), also named B23 or NO38, is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. NPM has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities. The NPM is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.
Product Overview
Entrez GenelD
4869
Aliases
B23; NPM
Clone#
7H10B9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Monkey
Immunogen
Purified recombinant fragment of human NPM (2-265) expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Morris SW, et al Science 1994 Mar 4; 263(5151):1281-4.
2. Yoneda-Kato N Oncogene 1996 Jan 18;12(2):265-75.
2. Yoneda-Kato N Oncogene 1996 Jan 18;12(2):265-75.
Product Image
Western Blot
Figure 1: Western blot analysis using NPM mouse mAb against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissues, showing nuclear localization using NPM mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Confocal Immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
For Research Use Only. Not for use in diagnostic procedures.