NS1 Primary Antibody
Item Information
Catalog #
Size
Price
Product Overview
Entrez GenelD
DQ357065.1
Aliases
Human parvovirus B19 isolate Vn115 NS1
Clone#
8E6E10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human Parvovirus NS1 (AA: 1-216) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
N
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using NS1 mAb against human Parvovirus NS1 (AA: 1-216) recombinant protein. (Expected MW is 33.4 kDa)
Western Blot
Figure 3:Western blot analysis using NS1 mAb against HEK293 (1) and NS1 (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using NS1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using NS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of K562 cells using NS1 mouse mAb (green) and negative control (red).
Flow cytometric
Figure 7:Flow cytometric analysis of Hela cells using NS1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.