NR6A1 Primary Antibody

Item Information
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Price
Description

This gene encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. The gene expresses at least alternatively spliced transcript variants.

Product Overview
Entrez GenelD
2649
Aliases
RTR; GCNF; NR61; GCNF1
Clone#
4G8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human NR6A1 (AA: 65-118) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/400
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Biochem Biophys Res Commun. 2010 Dec 10;403(2):178-83.
2.Cell. 2005 Sep 23;122(6):957-68.
Product Image
Western Blot
Figure 1: Western blot analysis using NR6A1 mAb against human NR6A1 recombinant protein. (Expected MW is 32.4 kDa)
Figure 1: Western blot analysis using NR6A1 mAb against human NR6A1 recombinant protein. (Expected MW is 32.4 kDa)
Western Blot
Figure 2: Western blot analysis using NR6A1 mAb against HEK293 (1) and NR6A1 (AA: 65-118)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using NR6A1 mAb against HEK293 (1) and NR6A1 (AA: 65-118)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using NR6A1 mouse mAb against K562 (1), NTERA-2 (2), HEK293 (3), HUVE-12 (4), and HeLa (5) cell lysate.
Figure 3: Western blot analysis using NR6A1 mouse mAb against K562 (1), NTERA-2 (2), HEK293 (3), HUVE-12 (4), and HeLa (5) cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of HepG2 cells using NR6A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 4: Immunofluorescence analysis of HepG2 cells using NR6A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 5: Flow cytometric analysis of K562 cells using NR6A1 mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of K562 cells using NR6A1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded cerebellum tissues using NR6A1 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded cerebellum tissues using NR6A1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NR6A1 mouse mAb with DAB staining.
Figure 7: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NR6A1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.