NPM3
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is related to the nuclear chaperone phosphoproteins, nucleoplasmin and nucleophosmin. This protein is strongly expressed in diverse cell types where it localizes primarily to the nucleus. Based on its similarity to nucleoplasmin and nucleophosmin, this protein likely functions as a molecular chaperone in the cell nucleus.
Product Overview
Entrez GenelD
10360
Aliases
PORMIN; TMEM123
Clone#
2A3B1
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human NPM3 (AA: full 1-178) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Nucleic Acids Res. 2012 Jun;40(11):4861-78.
2.Cell Chem Biol. 2018 Jan 18;25(1):110-120.e3.
2.Cell Chem Biol. 2018 Jan 18;25(1):110-120.e3.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using NPM3 mAb against human NPM3 (AA: full 1-178) recombinant protein. (Expected MW is 45.3 kDa)
Western Blot
Figure 3:Western blot analysis using NPM3 mAb against HEK293-6e (1) and NPM3(AA:full 1-178)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using NPM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hek293 cells using NPM3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using NPM3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NPM3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded mouse brain tissues using NPM3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded mouse kidney tissues using NPM3 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.