NPM2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
NPM2 (Nucleophosmin/Nucleoplasmin 2) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and enzyme binding. An important paralog of this gene is NPM1.
Product Overview
Entrez GenelD
10361
Clone#
1A6D4
Host / Isotype
Mouse / Mouse IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human NPM2 (AA: 1-214) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/100 - 1/400
ELISA
1/10000
References
1.Biochemistry. 2011 Sep 20;50(37):8078-89.
2.Curr Opin Struct Biol. 2003 Feb;13(1):6-14.
2.Curr Opin Struct Biol. 2003 Feb;13(1):6-14.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using NPM2 mAb against human NPM2 (AA: 1-214) recombinant protein. (Expected MW is 27 kDa)
Western Blot
Figure 3:Western blot analysis using NPM2 mAb against HEK293-6e (1) and NPM2 (AA: 1-214)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using NPM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded thyroid cancer tissues using NPM2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded human brain tissues using NPM2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.