NPC1 Primary Antibody

Item Information
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Price
Description

This gene encodes a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this gene cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by over accumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments.

Product Overview
Entrez GenelD
4864
Aliases
NPC
Clone#
8D10G3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human NPC1 (AA: 34-174) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Liver Int. 2010 Jul;30(6):887-97.
2.Neuroscience. 2010 May 19;167(3):608-20.
Product Image
Western Blot
Figure 1: Western blot analysis using NPC1 mAb against human NPC1 recombinant protein. (Expected MW is 37.6 kDa)
Figure 1: Western blot analysis using NPC1 mAb against human NPC1 recombinant protein. (Expected MW is 37.6 kDa)
Western Blot
Figure 2: Western blot analysis using NPC1 mAb against HEK293 (1) and NPC1 (AA: 34-174)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using NPC1 mAb against HEK293 (1) and NPC1 (AA: 34-174)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NPC1 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NPC1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using NPC1 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using NPC1 mouse mAb with DAB staining.
Flow cytometric
Figure 5: Flow cytometric analysis of Hela cells using NPC1 mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of Hela cells using NPC1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.