Notch3 Primary Antibody
Item Information
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Price
Description
This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
Product Overview
Entrez GenelD
4854
Aliases
IMF2; CASIL; CADASIL
Clone#
2E4D11
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human Notch3 (AA: 47-156) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1.Cancer Res. 2014 Jun 15;74(12):3282-93.
2.PLoS One. 2013 Dec 18;8(12):e84659.
2.PLoS One. 2013 Dec 18;8(12):e84659.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using Notch3 mAb against human Notch3 (AA: 47-156) recombinant protein. (Expected MW is 37.3 kDa)
Western Blot
Figure 3:Western blot analysis using Notch3 mAb against HEK293 (1) and Notch3 (AA: 47-156)-hIgGFc transfected HEK293 (2) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.