NGB
Item Information
Catalog #
Size
Price
Description
This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions.
Product Overview
Entrez GenelD
58157
Clone#
8C2H4
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human, Mouse, Rat
Immunogen
Purified recombinant fragment of human NGB(AA: full 1-151) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Zhongguo Dang Dai Er Ke Za Zhi. 2019 Jun;21(6):573-579.
2.Brain Behav. 2020 Mar;10(3):e01547.
2.Brain Behav. 2020 Mar;10(3):e01547.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using NGB mAb against human NGB (AA: full 1-151) recombinant protein. (Expected MW is 19.8 kDa)
Western Blot
Figure 3:Western blot analysis using NGB mAb against HEK293-6e (1) and NGB (AA:full 1-151)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using NGB mouse mAb against Rat liver (1), Mouse liver (2), Mouse brain (3) tissue lysate, and A549 (4),HUVEC (5),Hela (6),U251 (7), HepG2 (8) cell lysate.
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of HT-29 cells using NGB mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.