NGB

Item Information

Catalog #

Size

Price

Description

This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions.

Product Overview

Entrez GenelD

58157

Clone#

8C2H4

Host / Isotype

Mouse / Mouse IgG1

Species Reactivity

Human, Mouse, Rat

Immunogen

Purified recombinant fragment of human NGB(AA: full 1-151) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Zhongguo Dang Dai Er Ke Za Zhi. 2019 Jun;21(6):573-579.
2.Brain Behav. 2020 Mar;10(3):e01547.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using NGB mAb against human NGB (AA: full 1-151) recombinant protein. (Expected MW is 19.8 kDa)

Western Blot

Figure 3:Western blot analysis using NGB mAb against HEK293-6e (1) and NGB (AA:full 1-151)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using NGB mouse mAb against Rat liver (1), Mouse liver (2), Mouse brain (3) tissue lysate, and A549 (4),HUVEC (5),Hela (6),U251 (7), HepG2 (8) cell lysate.

Immunofluorescence analysis