NFKB2 Primary Antibody

Item Information
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Description

This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Product Overview
Entrez GenelD
4791
Aliases
p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100
Clone#
2F10A7
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human NFKB2 (AA: 712-900) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Blood. 2014 Nov 6;124(19):2964-72.
2.Mol Cancer Ther. 2013 Aug;12(8):1629-37.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using NFKB2 mAb against human NFKB2 (AA: 712-900) recombinant protein. (Expected MW is 45.9 kDa)
Figure 2:Western blot analysis using NFKB2 mAb against human NFKB2 (AA: 712-900) recombinant protein. (Expected MW is 45.9 kDa)
Western Blot
Figure 3:Western blot analysis using NFKB2 mAb against HEK293 (1) and NFKB2 (AA: 712-900)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using NFKB2 mAb against HEK293 (1) and NFKB2 (AA: 712-900)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using NFKB2 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using NFKB2 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.