NEFM Primary Antibody
Item Information
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Size
Price
Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Product Overview
Entrez GenelD
4741
Aliases
NFM; NEF3; NF-M
Clone#
2G12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human NEFM (AA: 381-443 ) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.FASEB J. 2010 Nov;24(11):4396-407. 2.J Biol Chem. 2005 Sep 9;280(36):31648-58.
Product Image
Western Blot
Figure 1: Western blot analysis using NEFM mAb against human NEFM recombinant protein. (Expected MW is 31.9 kDa)
Western Blot
Figure 2: Western blot analysis using NEFM mAb against HEK293 (1) and NEFM (AA: 381-443)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using NEFM mouse mAb against NTERA-2 (1), SK-N-SH (2), and PC-12 (3) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of SK-N-SH cells using NEFM mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.