NEFM Primary Antibody
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Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Western Blot

Figure 1: Western blot analysis using NEFM mAb against human NEFM recombinant protein. (Expected MW is 31.9 kDa)
Western Blot

Figure 2: Western blot analysis using NEFM mAb against HEK293 (1) and NEFM (AA: 381-443)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot

Figure 3: Western blot analysis using NEFM mouse mAb against NTERA-2 (1), SK-N-SH (2), and PC-12 (3) cell lysate.
Flow cytometric

Figure 4: Flow cytometric analysis of SK-N-SH cells using NEFM mouse mAb (green) and negative control (red).
Elisa

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.