NEFM Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesNFM; NEF3; NF-M
ProductOrderN
Clone#2G12
Entrez GenelD4741
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human NEFM (AA: 381-443 ) expressed in E. Coli.
MW102.4kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
381-443
Catalog#: 30411
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Images
Western Blot
Figure 1: Western blot analysis using NEFM mAb against human NEFM recombinant protein. (Expected MW is 31.9 kDa)
Figure 1: Western blot analysis using NEFM mAb against human NEFM recombinant protein. (Expected MW is 31.9 kDa)
Western Blot
Figure 2: Western blot analysis using NEFM mAb against HEK293 (1) and NEFM (AA: 381-443)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using NEFM mAb against HEK293 (1) and NEFM (AA: 381-443)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using NEFM mouse mAb against NTERA-2 (1), SK-N-SH (2), and PC-12 (3) cell lysate.
Figure 3: Western blot analysis using NEFM mouse mAb against NTERA-2 (1), SK-N-SH (2), and PC-12 (3) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of SK-N-SH cells using NEFM mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of SK-N-SH cells using NEFM mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

References (references)
References (references)1.FASEB J. 2010 Nov;24(11):4396-407. 2.J Biol Chem. 2005 Sep 9;280(36):31648-58.